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Explore an optimized sample-to-enrichment workflow for sequencing efficiency
As genomic studies scale in size and complexity, researchers need exome sequencing approaches that offer consistent, high-quality data without adding unnecessary time or cost. Variability in coverage, off-target sequencing, and workflow inefficiencies can compromise variant detection and slow progress, especially when working with challenging or GC-rich regions. DNA exome sequencing remains a powerful way to focus on the most biologically relevant regions of the genome but generally only when performance is predictable and reproducible.
The Applied Biosystems DNA Exome solution is a sample-to-enrichment workflow that offers high uniformity and low off-target rates, helping you achieve efficient sequencing and confident variant detection at scale.
Key benefits:
- High data confidence for sequencing efficiency—Excellent on-target rates, uniform coverage, and reliable detection of GC-rich and complex regions for more callable exome data per run
- Workflow efficiency saves on sequencing costs—Optimized for more samples per run, or deeper sequencing, using the same sequencing capacity
- Comprehensive and relevant coverage—>99% coverage of major coding databases, optimized for capturing historically challenging targets including SMAD4, TP53 exons, PTEN 5’ UTR gaps, and FGFR2 regions
- Quality you can trust—NGS QC performed on each lot of probes manufactured
Sequence more efficiently—starting with sample prep
Our sample-to-enrichment DNA exome workflow is designed to improve library quality and help you get more value from your sequencing runs.
Start by preparing DNA samples* using MagMAX nucleic acid extraction kits on the Thermo Scientific KingFisher Sample Purification System, with kit selection based on your sample type and application. Next, create NGS libraries using enzymatic fragmentation and library index adapters for consistent, reliable library preparation*. Targets can then be enriched using the NGS Exome DNA Panel, hybridization reagents, and capture beads to achieve uniform coverage across the exome.
After enrichment, libraries are ready to run on high-throughput next-generation sequencers and can be analyzed using commonly used bioinformatics tools.
This sample-to-enrichment workflow solution is designed to help you generate high-quality exome data with predictable performance—so you can focus on your research, not troubleshooting your workflow.
* Sample preparation and library preparation kits are also compatible with whole genome sequencing workflows.
Coverage uniformity: The double-stranded DNA (dsDNA) probe advantage
Applied Biosystems NGS capture solutions use double-stranded DNA probes for high on-target rates and uniform coverage across the exome. This approach supports reliable capture of GC-rich and complex regions, enabling confident variant detection with less sequencing. Combined with simplified, predictable workflows and rigorous NGS QC of all probes at manufacturing, laboratories can scale exome projects with confidence.
Uniform coverage reduces over and under sequencing, allowing you to get the data you need with less sequencing (Figures 1 and 2).
Figure 1. Data efficiency with uniform coverage. Uniformity (left panel) helps reduce waste by producing the right amount of data for optimized sequencing, while under- and over-sequencing misses critical data or provides extraneous data, causing sequencing waste (right panel).
Figure 2. Cost impact of improved uniformity. Total costs per run impacts compared to competitor DNA probes. Our enrichment kit helps save on downstream sequencing costs.
Talk to a sequencing workflow specialist to learn how the DNA Exome workflow can help you maximize data quality while optimizing sequencing spend.
Ordering information
Products for library prep and target enrichment
Workflow step |
Product |
Cat. No. |
|---|---|---|
Target enrichment |
NGS Exome DNA Panel, 2 Reactions (8‑Plex, 16 Samples) |
|
Target Enrichment Hybridize and Wash Kit with Amp Mix, 2 reactions (8‑Plex, 16 Samples) |
||
Quick Target Enrichment Hybridize and Wash Kit with Amp Mix, 2 reactions (8‑Plex, 16 Samples) |
||
Target Enrichment Universal Blockers, 2 reactions (8‑Plex, 16 Samples) |
||
Target Enrichment Binding & Purif Beads (Air Dry), 2 reactions (8‑Plex, 16 Samples) |
||
Library prep |
Enzymatic Fragmentation DNA Library Prep with Amp Mix, 16 Samples |
|
Library Index Adapter Kit, 16 samples |
Products for sample preparation depends on application and sample type
| Application | Common sample types | Product | Cat. No. |
|---|---|---|---|
| Hematology oncology | Whole blood, bone marrow | MagMAX DNA Multi-Sample Ultra 2.0 Kit | A36570 |
| MagMAX Sequential DNA/RNA Kit | |||
| Genomics | Biofluids, cells, tissue, saliva, swabs | MagMAX DNA Multi-Sample Ultra 2.0 Kit | A36570 |
| MagMAX HMW DNA Kit (up to 100 preps for fresh or frozen blood) | A36626 | ||
| MagMAX HMW DNA Kit (up to 100 preps for tissue or cells) | |||
| Solid tumors | FFPE, fresh/frozen tissue | MagMAX DNA Multi-Sample Ultra 2.0 Kit | A36570 |
| MagMAX FFPE DNA/RNA Ultra Kit** | A31881 | ||
| Liquid biopsy | Plasma, serum, urine | MagMAX Cell-Free DNA Isolation Kit | A29319 |
| MagMAX Cell-Free Total Nucleic Acid Isolation Kit | A36716 |
**Pair with AutoLys M Tubes for easy deparaffinization without the need for harsh chemical solvents
For Research Use Only. Not for use in diagnostic procedures.