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Streamline RNA detection with 1-Step RT-qPCR solutions
1-step RT-qPCR master mixes enable efficient RNA detection across gene expression, microbial detection and surveillance, microRNA analysis, and multiplex research workflows. By combining reverse transcription and real-time PCR in a single reaction, these solutions help simplify setup, accelerate RNA-to-result turnaround, and support consistent, scalable performance in high-demand laboratory environments.
Designed to support:
- Up to 45% fewer setup steps
- Up to 82% reduction in total RT-qPCR time
- Closed-tube workflow design to help minimize contamination risk
1-Step RT-qPCR master mix solutions
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Order master mixes |
4391178—100 reactions 4389986—500 reactions |
Rox 4444432—200 reactions 4444434—1,000 reactions 4444436—2,000 reactions |
No Rox 5555534—1,000 reactions 5555536—2,000 reactions |
Ideal for |
Gene expression | Microbial detection Gene expression |
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Cycling mode: run time |
Standard: ~90 minutes | Fast: ~27 minutes | |
Multiplexing |
Single-target detection | Up to 3-plex (Rox) Up to 4-plex (No Rox) |
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Key features |
SYBR chemistry Melt curve compatible Low-abundance sensitivity Two-tube format Widely used production controls |
Probe-based chemistry Fast protocol with high sensitivity Inhibitor tolerant Single-tube format |
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Order with rox or no rox | ||
1-Step TaqPath master mix solutions
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Order master mixes |
With mustang purple
A28525—100 reactions A28526—1,000 reactions A28527—2,000 reactions |
No Rox
A28521—100 reactions A28522—1,000 reactions A28523—2,000 reactions |
Rox
A58666—100 reactions A58667—1,000 reactions A58668—2,000 reactions |
No Rox
A58669—100 reactions A58670—1,000 reactions A58671—2,000 reactions |
Ideal for |
Multiplex gene expression Assay development |
Automated RT-qPCR workflows Advanced multiplex Inhibitor-rich samples Complex matrices |
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Cycling mode: run time |
Standard: ~36 minutes | Standard: ~34 minutes |
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Multiplexing |
Up to 4-plex | Up to 5-plex (Rox) Up to 6-plex (No Rox) |
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Key features |
Probe-based chemistry Reproducible sensitivity Single-tube format |
Enhanced inhibitor tolerance & low copy detection Single-tube format Up to 8 hours benchtop stability |
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Order with mustang purple or no rox | Order with rox or no rox | ||
The Taqpath master mixes are manufactured in an ISO 13485-certified and FDA-registered facility that adheres to the principles of current good manufacturing practices (cGMP).
1-Step master mix solutions are compatible with Applied Biosystems TaqMan assays and QuantStudio real-time PCR systems.
Proven performance in multiplex and challenging samples
Consistent multiplex performance is critical for complex sample matrices, inhibitor-rich specimens, and low copy number targets. TaqPath DuraPlex 1-Step RT-qPCR Master Mix is designed to support reliable detection in advanced, high-complexity workflows where precision and scalability matter.
RT-qPCR resources
Whether you’re optimizing multiplex assays or enhancing RNA detection workflows, these qPCR resources offer practical insights to support confident experimental design.
Understand the differences between one-step and two-step real-time PCR including workflow design, flexibility, sample handling, and experimental considerations. This resource helps guide selection of the appropriate RT strategy based on application needs and laboratory workflow priorities.
1-step qPCR master mixes for qPCR research workflow applications
Comprehensive solutions for your RT-qPCR workflow
Complete your RT-qPCR workflow with sample preparation kits, assay solutions, and compatible consumables designed to support efficient and reliable RNA detection from extraction to analysis.
RNA & nucleic acid isolation
qPCR consumables
1-Step qPCR FAQs
Explore answers to key questions about 1-Step qPCR workflow efficiency, multiplex strategies, contamination control, and performance considerations.
A 1-step RT-qPCR master mix combines reverse transcription and real-time PCR amplification in a single reaction tube, enabling RNA-to-result workflows without transferring cDNA between steps. Solutions such as TaqMan Fast Virus 1-Step master mix and TaqPath DuraPlex 1-Step RT-qPCR master mix are designed to simplify setup while supporting sensitive RNA detection across gene expression, microbial detection, and multiplex applications.
In 1-step RT-qPCR, reverse transcription and amplification occur in the same tube. In 2-step RT-qPCR, cDNA synthesis is performed separately before PCR. The 1-step approach, used in solutions such as TaqMan 1-Step Multiplex master mix, can reduce handling steps and consolidate reactions into a single setup. Learn more in the RT-qPCR Learning Center.
1-step RT-qPCR simplifies workflow by combining reverse transcription and amplification in a single reaction, reducing setup steps and sample handling. The closed-tube format helps minimize contamination risk. Solutions such as TaqMan Fast Virus 1-Step Master Mix and TaqPath DuraPlex 1-Step RT-qPCR Master Mix support sensitive RNA detection.
For microbial detection, including viral and bacterial RNA targets, a probe-based 1-step RT-qPCR master mix optimized for sensitivity and inhibitor tolerance is typically recommended. TaqMan Fast Virus 1-Step master mix supports rapid viral detection, while TaqPath DuraPlex 1-Step RT-qPCR master mix is designed for multiplex pathogen panels and complex sample matrices.
For multiplex RT-qPCR, choose a master mix optimized for multi-target detection and compatible dye configurations. TaqMan 1-Step Multiplex master mix supports up to 4-plex workflows, while TaqPath DuraPlex 1-Step RT-qPCR master mix is designed for higher-order multiplexing and challenging samples requiring enhanced inhibitor tolerance.
By combining reverse transcription and amplification into one reaction, 1-step RT-qPCR workflows can reduce total experiment time. In some protocol comparisons, total RT-qPCR time may be reduced by up to 82%, depending on instrument and assay conditions. Fast-cycle master mixes such as TaqMan Fast Virus 1-Step master mix are designed to support accelerated turnaround.
1-step RT-qPCR reduces hands-on steps by combining reverse transcription and amplification in a single reaction, eliminating the need for cDNA transfer. In some workflows, setup steps may be reduced by up to 45%. Single-tube formats, such as those used in TaqPath DuraPlex 1-Step RT-qPCR Master Mix, support simplified workflow design.
For Research Use Only. Not for use in diagnostic procedures.