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Mass Spectrometry Sample Clean-Up Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
Getting Started
- What kind of substances in my sample would interfere with mass spectrometry analysis?
- What is the best way to remove small contaminants from my sample for mass spectrometry analysis?
- Can I use protein desalting columns for cleanup of peptide samples?
- What is the best way to remove detergents from my sample for mass spectrometry analysis?
- What kind of buffers and solutions are compatible with peptide clean-up and LC-MS?
Troubleshooting
- I cleaned up my peptide sample using C18 or desalting columns, but I lost most of my peptides. What went wrong?
- I still have detergent present in my sample after protein digestion. Is there anything I can do?
- I have a low number of protein identifications from my protein digest sample. What can I do?
- I’m seeing a peak in my mass spectrometry analysis results that seems to always be present in my system. What can I do?
- I’m seeing extra peaks in my mass spectrometry chromatogram that do not appear to come from my protein sample or the system. Where did they come from and what should I do?
Guides and Tools
- Overview: Sample Prep for Mass Spectrometry
- Overview: Detergent Removal from Peptides
- Overview: C18 Columns & Peptide Desalting for Mass Spectrometry
- Overview: Abundant Protein Depletion for Mass Spectrometry
- Overview: Peptide Enrichment and Fractionation for Mass Spectrometry
- Overview: Protein Enrichment & Clean Up for Mass Spectrometry
- Overview: Protein Dialysis, Desalting, Concentrators, and Small Molecule Removal
- Overview: Plasma Protein Binding Equilibrium Dialysis
- Guide: Workflow for Protein Mass Spectrometry
- Handbook: Protein Sample Preparation and Quantitation for Mass Spectrometry
- DailyCalcs Science Calculator : An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a transfection calculator you can use to simplify transfection experimental setup.
Custom Services
Custom Peptide Synthesis Services
Thermo Scientific Custom Peptide synthesis service offers numerous options for synthesis platforms, purity levels, modifications, and formats to meet a variety of research needs. We synthesize peptides of 2–110 amino acids using the latest FMOC solid-phase technology, and purify our products using HPLC. Synthesis scales range from 0.1 mg to 1 g, and purities from crude to >98% based on the service.
HeavyPeptide AQUA Custom Synthesis Service
Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labeled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures. Peptides are synthesized using the latest FMOC solid-phase technology and purified by HPLC. All HeavyPeptide sequences come with mass spectrometric analysis and analytical HPLC dat. Additionally, we offer a wide range of labels, modifications, scales, and purities.
Bulk and Custom Protein Biology Products
Partner with us to scale up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications. Whether you are developing an assay for commercialization, constructing a large screening assay for in-house use, or requiring high-purity reagents for your manufacturing process, we can provide consistent quality in milligram to kilogram quantities manufactured under ISO 9001 processes. Complete our bulk and custom products request form to get your project started.
For Research Use Only. Not for use in diagnostic procedures.