Gibco Cell Culture Media for Bioprocessing

Gibco CHO media for scalable performance

Our CHO media offerings help maintain growth and consistent protein expression when working with Chinese hamster ovary (CHO) cells. Each formulation is designed to support process efficiency and smooth transition from development to large-scale manufacturing. By strengthening control and yield across workflows, these media help provide confidence in performance and reproducibility.

Gibco Dynamis Medium

Built for performance and consistency, Dynamis Medium supports higher titers and fast process development across CHO and HEK293 workflows. Its adaptable formulation enables growth and simplified scale-up, helping achieve reliable yield and efficiency from development to production.
 

Gibco Efficient-Pro Medium and Feeds System

The Efficient-Pro Medium and Feeds System facilitates high-density CHO cell growth and reliable protein expression through flexible feeding strategies. The platform enables fast optimization, consistent performance, and efficient scale-up to help streamline workflows from development to manufacturing.
 

Gibco High-Intensity Perfusion Medium

High-Intensity Perfusion Medium supports continuous, high-density CHO cell growth and sustained productivity in perfusion processes. Its optimized formulation helps extend culture longevity and maintain product quality, enabling efficient, scalable bioprocessing with consistent results from development to commercial scale.

Support consistent cell growth with Gibco cell culture media across multiple formats

Gibco cell culture media are developed for consistent, high-performance growth environments across formats, including liquid media, dry powder media (DPM), and Advanced Granulation Technology (AGT). Each format supports scalable workflows and reliable results, from process development through manufacturing, helping teams maintain efficiency and quality across applications.

AGT

AGT media helps streamline preparation and consistent performance across bioprocessing workflows. The dry, granulated format supports reproducible hydration, improved solubility, and reduced handling steps, helping uphold efficiency and maintain control from development through scale-up.

Diploid growth and production media

Diploid growth and production media support consistent vaccine development by minimizing process variability and exposure to serum. These formulations enable reliable cell growth and viral yield across manufacturing stages. The result is streamlined production with greater performance confidence.

Virus production media and reagents

Gibco virus production media and reagents enhance viral growth and yield while minimizing the use of serum in vaccine development. These formulations accommodate scalable production and controlled infection conditions, preserving reproducibility from research through commercial manufacturing.

Choose the right media format for your workflow

Selecting the appropriate cell culture media helps align process needs with operational efficiency and quality goals. Gibco media are available in multiple formats to support scalability, simplify preparation, and preserve performance across workflows.

 

Cell culture media formats and specifications

Format

Description

Volume sizes

Package sizes (Approximate max capacity)

AGT

Dry granulated format that simplifies handling and reconstitution while meeting storage and quality-control requirements. Available across Gibco media and feeds.

1–1,250 kg
  • Securitainers: 1–25 L*
  • Ropack Buckets: 120–600 L*
  • HDPE Drums: 4 × 600 L to 6 × 1,000 L*+

Liquid media

Liquid format for performance media, feeds, and buffers. Supports flexible BioProcess Containers (BPCs) from development to production.

1–10,000 L
  • PET Bottles: 0.1–1 L
  • HDPE Bottles: 0.1–1 L
  • Bags: 1–200 L

DPM

Standard dry powder for performance media, feeds, and additives in bioprocessing.

1–7,500 kg
  • Securitainers: 1–35 L*
  • Ropack Buckets: 150–800 L*
  • HDPE Drums: 4×600 L to 6×1000 L*+

* Assuming 23 g/L reconstitution ratio.

+ Each drum contains multiple bags of medium.

Discover other cell culture solutions to scale processes

From early process development to large-scale manufacturing, Gibco cell culture solutions help enable reliable, flexible workflows across biotherapeutic applications. For more than 60 years, these technologies have supported scalable and efficient production, maintaining performance and consistency. Explore complementary sera, supplements, and reagents designed to help improve your workflow.

Cell culture sera

Gibco cell culture sera are formulated for consistent performance and helps reduce process risk. Sourced from regions keeping reduced BSE risk in mind and processed under cGMP-compliant conditions, each lot enables traceability and configuration options to align with diverse bioprocessing needs.


 

Feeds and supplements

Gibco feeds and supplements help optimize cell growth and productivity across CHO and other cell lines. With options including Gibco EfficientFeed+ supplements, Gibco GlycanTune total feeds, and AGT dry-granular formats, these solutions are formulated to enhance workflow flexibility and preparation efficiency from development through production.

 

Functional additives and reagents

Gibco functional additives and reagents optimize workflows by supporting reliable performance during process development. From animal origin-free (AOF) formulations to process enhancers, these solutions enable flexibility and reproducibility across cell culture applications.

Stable cell line development kits

Our stable cell line development kits streamline the transition from transient expression to stable production. Built for consistency, scalability, and high performance, they enable efficient, reproducible workflows for modern biotherapeutic manufacturing.


 

Frequently asked questions

Cell culture media vary by composition and purpose, including basal media that supply nutrients, serum-free formulations for defined conditions, and specialized media for cell types such as CHO or HEK293. Each format is designed to maintain steady growth and reproducibility across applications.

Dulbecco’s Modified Eagle Medium (DMEM) and Minimum Essential Medium (MEM) differ in nutrient concentration and formulation. DMEM includes higher levels of amino acids, glucose, and vitamins for metabolically active cells, while MEM contains a simpler mix suited for less demanding cultures.

Shelf life varies depending on the formulation and storage conditions. Liquid media typically perform reliably for six to 12 months under recommended conditions, while dry formats such as AGT and DPM can remain stable for longer periods. Check the expiration date on each lot for consistent results throughout its intended use.

The right format depends on process scale, storage needs, and workflow design. Liquid media offer convenience for small- to mid-scale use, while AGT and DPM formats support larger production volumes and extended storage. Each option helps maintain consistency and control across bioprocessing steps to help match performance with operational goals.

Thermo Fisher Scientific supports custom media development to meet specific process or cell line requirements. Our Media Customization Services team collaborates with customers to develop formulations, concentrations, and packaging formats for specific production requirements.

Cell culture resources

Access practical tools, technical guides, and learning materials to support process development and optimization. The Cell Culture Resource Hub connects you with educational content, workflow insights, and product documentation to help maintain consistency and efficiency across applications.

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.