Gibco Efficient-Pro Media and Feeds System

The Efficient-Pro System is designed to help improve workflows across upstream mAb processes by pairing a chemically defined medium with feeds that offer controlled nutrient delivery. This approach helps maintain cell growth and protein expression in fed-batch cultures. The formulations support reliable titer performance and can reduce variation between runs. Because the system is compatible with common CHO cell lines, it can also help facilitate a smoother transition from development to scale-up.

The Efficient-Pro System is suitable for use with CHO-K1, CHO-S, DG44, and CHO-K1 GS cell lines. Its medium and feed formulations are developed to help maintain cell growth and protein expression across these systems. Each feed option supports nutrient delivery patterns customized to different cell line needs, which can help reduce variation in fed-batch cultures. This flexibility allows the system to be incorporated into a range of upstream development and scale-up activities.

AGT media offer a practical alternative to traditional liquid or powdered formats. Because the granules dissolve easily, preparation can be completed with fewer mixing steps. Their uniform composition allows each batch to reconstitute. The dry format helps reduce storage needs and is straightforward to handle during routine upstream work, making it suitable for use in development and production settings.

Efficient-Pro Medium (+) Insulin, Feed 3, and the Feed Enhancer are formulated to integrate into standard fed-batch workflows without needing to change established methods. The pre-supplemented medium can minimize the need for separate insulin additions, while Feed 3 and the Feed Enhancer offer nutrient support for CHO-K1 GS cultures. Each component is prepared using familiar steps, allowing teams to introduce them into ongoing development or scale-up activities with minimal adjustment. Together, these options help maintain consistent culture performance across process stages.

The Efficient-Pro System is manufactured using chemically defined animal origin–free components to support performance from development through production. Its documentation package is designed to meet the needs of regulatory review and commercial manufacturing. The medium and feeds are available in formats for larger-scale operations, allowing teams to transition processes without altering core upstream conditions. These features help maintain continuity as workflows advance toward cGMP production.