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Optimize scale-up of CHO cell lines
Scaling Chinese hamster ovary (CHO) cell cultures can reveal challenges such as uneven growth, nutrient shifts, and lot-to-lot variability that affect yield and quality. Gibco Dynamis Medium is designed to improve yield with a chemically defined animal origin-free (AOF) formulation that supports high-density suspension CHO systems. Manufactured under cGMP conditions, Dynamis Medium has been applied in bioprocesses that target consistent metabolism and protein expression across various scales. The Gibco Efficient-Pro Medium and Feeds System can facilitate cell health maintenance, improved yield, and reduced variability from development through production.
Benefits of Gibco Dynamis Medium
Formulated for use in upstream applications, Dynamis Medium is used in processes focused on productivity and streamlined process transfer. Its balanced formulation supports predictable growth and reliable protein expression during culture scale-up.
Dynamis Medium is formulated to:
- Promote high cell density and sustained viability in fed-batch processes
- Facilitate consistent productivity across CHO and HEK293 cultures
- Enable faster process development and has been evaluated for titer performance
- Simplify preparation and storage with liquid and Advanced Granulation Technology (AGT) dry formats
Optimize growth across CHO cell lines
Building on its balanced formulation and cGMP-quality manufacturing, Dynamis Medium supports reliable performance across common CHO cell lines, including DG44, CHO-K1, CHO-GS, and CHO-S. The medium is formulated to sustain cell metabolism and viability in batch and fed-batch systems, enabling consistent growth and titer performance through late-stage culture. When used with Gibco EfficientFeed A+ Supplement, Gibco EfficientFeed B+ Supplement, or Gibco EfficientFeed C+ Supplement, its controlled nutrient profile supports stable expression and reproducibility during clone selection, process development, and scale-up.
Ordering information
Support efficient cell culture with CHO media
As CHO cultures move from development to production, media selection plays a central role in maintaining cell health and protein expression. Gibco CHO media are designed for flexibility across fed-batch and perfusion formats, helping process scientists manage nutrient balance and reproducibility across scales.
Explore other media for consistent cell growth
Beyond CHO-based processes, Gibco cell culture media are designed to support consistent performance across multiple cell types and production systems.
Frequently asked questions
Variability in growth and productivity can make process efficiency and scale-up more difficult to predict. Factors such as nutrient composition, feed timing, and adaptation between cell lines often contribute to these differences. Gibco Dynamis Medium has been evaluated in CHO-K1, CHO-S, and DG44 cultures for cell density and steady protein expression. In combination with Gibco Efficient Feed Supplements, consistent growth profiles have been observed across development and production scales.
Differences between development and production stages often reflect changes in nutrient levels, mixing, and oxygen transfer that occur as volumes increase. These factors can influence cell metabolism and growth characteristics across scales. Variations in feed delivery or aeration may also affect overall process consistency. Using comparable media and feed formulations at both scales can reduce variability in culture behavior.
In studies using Gibco Dynamis Medium, HEK293 cells were seeded at 0.3 × 10⁶ cells/mL with 4 mM L-glutamine and cultured in 60 mL shake flasks under standard conditions. Viable cell-density measurements through day 7 indicated consistent, high-density growth; cultures required glucose supplementation by day 4 or at inoculation to maintain performance.
For CHO applications, Dynamis Medium was evaluated with internal IgG-producing CHO DG44 and CHO K1 lines in fed-batch experiments lasting up to 21 days. Cultures supplemented with EfficientFeed C+ maintained viability and productivity through late stages. Across studies, Dynamis Medium supported steady cell growth and sustained viable cell densities under fed-batch conditions.
Feed selection depends on cell line behavior, culture duration, and production goals. EfficientFeed A+, B+, and C+ supplements are commonly used with Dynamis to manage nutrient availability during fed-batch operation. These formulations differ in composition and feeding profiles, allowing evaluation based on process requirements. Gibco FunctionMAX TiterEnhancer can also be assessed as part of feed and process optimization studies.
Transitioning from existing media to Dynamis Medium typically begins with small-scale parallel studies to compare growth and productivity relative to current formulations. Stepwise adaptation can be used to evaluate cell response and assess culture stability. Direct use can be evaluated if process parameters are comparable. Results from these evaluations help define feeding schedules, timing, and process adjustments during larger-scale implementation.
Regulatory readiness is based on transparent documentation and traceability across manufacturing sites operating under cGMP conditions. Certificates of analysis, lot traceability, and documentation packages are available for qualification and filing needs. Information on raw materials and manufacturing controls can be shared through established request processes for use in regulatory submissions and audits.
Feed and media evaluations can identify conditions that influence growth, viability, and product quality. Comparative testing across formulations enables analysis of nutrient composition, feed strategy, and culture performance over time.
Additional bioprocessing resources
Bioprocessing resources
