Diploid Cell Culture Media for Viral Vaccine Manufacturing

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Support scalable diploid cell growth for vaccine manufacturing

Reliable diploid cell growth supports consistent vaccine production. Gibco diploid cell media are designed to maintain healthy attachment and viability across diploid lines such as MRC-5, WI-38, KMB17, and chicken embryo fibroblasts (CEF). As you move from serum-based to reduced-serum or animal origin–free (AOF) workflows, these formulations help reduce process risk and support easy adaptation from development to large-scale cGMP production.

Benefits of diploid cell media

Gibco diploid cell media facilitate consistent performance across vaccine development and production. Each serum-reduced, chemically defined, and animal origin–free formulation supports workflow efficiency, process control, and reproducibility.

Some benefits include:

  • Reduced contamination risk
  • Simplified regulatory documentation
  • Improved process consistency
  • Enhanced scalability 
  • Reliable performance across sites


Streamline vaccine production with diploid cell culture


Efficient workflows are critical for consistent viral vaccine production. Diploid cell media are designed to help simplify preparation and reduce process variability while supporting reliable growth and virus expression. Serum-reduced and animal origin–free formulations allow for smooth adaptation across development and manufacturing, helping minimize the need for extensive process changes. By maintaining high cell viability and predictable virus yield, these media can accelerate production timelines and strengthen regulatory confidence across cGMP environments.

Ordering information

货号 产品名称 规格 Price 数量
A3968901 Diploid Growth Serum-Reduced Medium (SRM), 10 L Each
9,852.00

货号 产品名称 规格 Price 数量
A3969002 Diploid Production Serum-Free Medium (SFM), 100 L Each
81,142.00

Explore other media for consistent cell growth

Thermo Fisher Scientific offers Gibco cell culture media that enable consistent performance across applications beyond diploid systems. Available in liquid, dry powder, and Advanced Granulation Technology (AGT) formats, these media seek to simplify preparation and help maintain reproducibility across sites.

CHO media


Gibco CHO media support cell growth and protein expression across scales, from development to manufacturing. Each formulation promotes consistent performance, helping you maintain productivity and quality in bioprocessing workflows.

Virus production media and reagents

Gibco virus production media and reagents are designed to support efficient viral growth and yield while minimizing variability in vaccine manufacturing. Each formulation enables scalable processes that maintain consistency from research through commercial production.

Explore end-to-end vaccine bioprocessing solutions

Thermo Fisher offers integrated solutions connecting upstream and downstream vaccine manufacturing. From cell expansion and virus expression to purification, each step is backed by reliable technologies and technical experience. These connected workflows promote improved scalability and simplify qualifications in cGMP environments. With global manufacturing and supply capabilities, Thermo Fisher helps vaccine producers maintain quality and consistency from development through commercial scale.
 

Discover other cell culture solutions to scale vaccine processes

Scaling vaccine production requires consistent performance and flexible tools across upstream and downstream workflows. Thermo Fisher supports these needs with a wide range of cell culture solutions designed to help optimize yield, control variability, and maintain reproducibility across sites. Explore complementary products such as feeds and supplements, functional additives and reagents, and bioreactors developed to strengthen control and efficiency throughout your process.
 

Find the right media formulation for your process

Every vaccine manufacturing process has specific performance requirements. Thermo Fisher can work with you to design custom Gibco media formulations that align with defined cell lines, culture conditions, and production goals. Our specialists can collaborate with you to optimize nutrients, components, and concentrations for scalable, cGMP-ready workflows. These tailored formulations help improve process consistency, support regulatory documentation, and enable a smooth transition from development to commercial scale.
 

Frequently asked questions

Diploid cell lines such as MRC-5 and WI-38 are used in viral vaccine production because they retain standard chromosome structure and consistent growth. Their similarity to primary human cells supports efficient virus replication and reliable yield. Over decades of use, these cells have shown strong safety records and are well accepted by regulators for vaccine manufacturing.

Serum-based media can introduce challenges, such as variability, that can affect reproducibility and viral yield. Lot-to-lot differences alter nutrient composition, which can change cell behavior and virus expression. Serum can also increase contamination risk and complicate documentation for regulatory submission.

Serum-reduced and AOF diploid media can help improve process consistency by reducing undefined serum components that cause variability. With defined formulations, cells can receive uniform nutrients at every stage, supporting reproducible growth and virus yield. These media also lower contamination risk and simplify qualification, helping maintain consistent performance across cGMP manufacturing sites.

Gibco diploid cell medium is designed for use in cGMP manufacturing environments. Its defined compositions support traceability, documentation, and lot-to-lot consistency needed for regulated vaccine production. These attributes help manufacturers maintain quality control and reproducibility across global sites.

Viral vaccine resources

Access resources to support every stage of viral vaccine production. The Viral Vaccine Resource Hub offers application notes, workflow guides, and case studies focused on cell culture optimization, virus expression, and purification.

 

Enable accelerated vaccine development with diploid media

For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.