Gibco High-Intensity Perfusion CHO Media

Continuous perfusion requires controlled nutrient delivery and stable cell behavior over long culture periods. HIP CHO Medium is formulated to help maintain these conditions by supplying nutrients at levels that support growth at low VVD exchange rates. In extended runs, cultures have shown steady viability with limited shifts in metabolism. These characteristics help support high-density operation under continuous perfusion conditions.

HIP CHO Medium can be evaluated in N-1 and intensified fed-batch formats where cultures require controlled growth and stable viability at higher cell densities. Its component profile supports cell expansion under conditions with increased passage frequency or elevated starting densities. In these applications, nutrient availability and metabolic behavior remain important factors to monitor as cultures approach higher cell concentrations. Results from internal studies indicate that HIP CHO Medium can maintain growth and viability in these intensified formats when process parameters are optimized to align with perfusion-based conditions.

HIP CHO Medium has been evaluated with several commonly used CHO hosts, including CHO-S, CHO-K1, CHO-GS, and DG44. Across these systems, cultures have shown stable growth and viability under perfusion conditions when operated at low VVD exchange rates. Differences in behavior between cell lines may require adjustment of seeding density, retention strategy, or operating parameters. In general, each of these CHO platforms has demonstrated consistent performance when paired with process settings suited to perfusion operation.

HIP CHO Medium is designed for continuous operation, where nutrients are supplied at low exchange rates and cells remain in culture for extended periods. Standard batch-fed formulations are typically optimized for periodic nutrient additions and shorter culture duration. These differences influence how cells respond to nutrient levels, byproduct accumulation, and overall metabolic conditions. In perfusion testing, HIP CHO Medium has supported steady viability and controlled growth under conditions that differ from those used in fed-batch operation.

HIP CHO Medium supports the perfusion stage of CHO processes by maintaining growth and viability under continuous operation. In a broader workflow, it accompanies upstream media, such as Gibco Dynamis Medium and the Efficient-Pro Medium and Feeds System, used in cell expansion and fed-batch development. These components can be evaluated together to manage nutrient needs as cultures progress toward higher densities. HIP CHO Medium offers a perfusion-specific option that aligns with scalable upstream strategies.

HIP CHO Medium is manufactured under cGMP conditions with controls that help support lot-to-lot consistency and documentation needs. The formulation is animal origin–free (AOF), which reduces variability associated with raw materials and supports regulatory expectations for upstream materials. Production is supported by Thermo Fisher Scientific's global supply network, which helps maintain reliable availability across regions. These factors contribute to consistent performance as processes move from development to clinical and commercial stages.