Esterase substrates for cell viability studies—Table 15.1 | Thermo Fisher Scientific - CN

Table 15.1 Esterase substrates for cell viability studies.

Esterase Substrate (Cat. No.)	Properties in Cells	pK_a of Product *
BCECF AM (B1150, B1170, B3051)	Quite well retained Released during cytolysis pH-sensitive fluorescence	7.0
Calcein AM (C1430, C3099, C3100MP)	Quite well retained Released during cytolysis Not as pH-sensitive as BCECF	5
Carboxyeosin diacetate, succinimidyl ester (CEDA, SE; C22803; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4)	Well retained by reaction with amines Useful for DAB photoconversion Phosphorescent	<5 †
Carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA, C369)	Moderately well retained Not as pH-sensitive as CFDA	4.8
Carboxyfluorescein diacetate (5(6)-CFDA, C195)	Moderately well retained pH-sensitive fluorescence	6.4
Carboxyfluorescein diacetate, acetoxymethyl ester (5-CFDA, AM; C1354)	Easier to load than CFDA yet yields the same product upon hydrolysis pH-sensitive fluorescence	6.4
Carboxyfluorescein diacetate, succinimidyl ester (5(6)-CFDA, SE; C1157; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4)	Well retained by reaction with amines Not completely released during cytolysis pH-sensitive fluorescence	6.4 †
CellTracker Green CMFDA (CMFDA; C2925, C7025)	Well retained by reaction with thiols Not completely released during cytolysis pH-sensitive fluorescence	6.4 †
Chloromethyl SNARF-1, acetate (C6826)	Well retained by reaction with thiols Not completely released during cytolysis Long-wavelength, pH-sensitive fluorescence	7.5 †
Fluorescein diacetate (F1303)	Poorly retained pH-sensitive fluorescence Inexpensive	6.4
Oregon Green 488 carboxylic acid diacetate (carboxy-F₂FDA, O6151)	Moderately well retained Not as pH-sensitive as CFDA	4.7
* Approximate pK_a values in aqueous solvents. The actual pK_a of the indicator will vary somewhat depending upon experimental conditions. † pK_a of the unconjugated hydrolysis product; after conjugation to an intracellular amine or thiol, the actual pK_a value may be different.

仅供科研使用，不可用于诊断目的。