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Brilliant Ultra Violet™ 661 Dye
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| 4 | 355 | 670/25, 630 LP | 349 | 659 | (in buffer) 3 | flow cytometry |
Invitrogen Brilliant Ultra Violet™ 661 dye (BUV661) is a tandem dye excited by the 349 nm UV laser and emits at 659 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining. BUV661 may have some cross-laser excitation with the red laser.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer BUV661 dye conjugated to primary antibodies for use in flow cytometry.
Additional products
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Spectral signature of Brilliant Ultra Violet™ 661 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 661 were used for analysis.
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Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet™ 661. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation
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Cell surface staining of C57BL/6 mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet™ 661 dye (left) or 0.25 µg of CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 661 dye (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.
Brilliant Ultra Violet dye background
Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.
Cy™ 为 GE Healthcare 的商标或注册商标。
不得转售。Super Bright 聚合物染料依据 Becton, Dickinson and Company 的许可销售。
Brilliant Ultra Violet™ 与 Brilliant Violet™ 为 Becton, Dickinson and Company 或其附属公司的商标或注册商标，经许可使用。
仅供科研使用。不可用于诊断目的。