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NovaFluor Blue 555 Dye
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| 2 | 488 | 542/18 | 493 | 555 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Blue 555 dye is a yellow-green fluorescent dye with an excitation and emission profile similar to Alexa Fluor 532 dye, but is 2.5 times brighter. This dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. NovaFluor 555 dye can be used in flow cytometry panels that include both NovaFluor Blue 510 and NovaFluor Blue 530 dyes.
The macromolecule-based NovaFluor Blue 555 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Can serve as a brighter replacement for Alexa Fluor 532 dye
- Can be used in a flow cytometry panel with NovaFluor Blue 510 and NovaFluor Blue 530 dyes
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Blue 555 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 555 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Blue 555 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD19 Monoclonal Antibody, NovaFluor Blue 555 (right). Data was acquired in the B3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor dyes
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Cy™ 为 GE Healthcare 的商标或注册商标。
不得转售。Super Bright 聚合物染料依据 Becton, Dickinson and Company 的许可销售。
Brilliant Ultra Violet™ 与 Brilliant Violet™ 为 Becton, Dickinson and Company 或其附属公司的商标或注册商标，经许可使用。
仅供科研使用。不可用于诊断目的。
Stylesheet for Classic Wide Template adjustments
Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标，经许可使用。
仅供科研使用，不可用于诊断目的。