NovaFluor Blue 510荧光染料-赛默飞 | Thermo Fisher Scientific

Novafluor Blue 510 excitation shown as dotted line and emission shown as solid green histogram


2	488	506/18	496	511	(in buffer) 5	flow cytometry

NovaFluor Blue 510 dye can be used as an alternative to BD Horizon Brilliant Blue (BB515) with a similar excitation spectrum and a cleaner emission spectrum. For example, in flow cytometry panels that include both FITC and BB515, replacing BB515 with NovaFluor Blue 510 dye reduces the complexity score, thus simplifying spectral unmixing of the panel. NovaFluor Blue 510 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. With distinct spectral signatures, NovaFluor Blue 510, NovaFluor Blue 530, and NovaFluor Blue 555 dyes can be used together in a flow cytometry panel. The macromolecule-based NovaFluor Blue 510 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.

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Experimental data using NovaFluor Blue 510 dye

Graph of relative intensity vs wavelength for NovaFluor Blue 510 dye

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 510 dye.

Graph of fluorescence intensity vs channel for NovaFluor Blue 510 dye

Figure 2. Spectral signature of NovaFluor Blue 510 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 510 dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.

Side scatter plot with unstained and CD16 NovaFluor Blue 510-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, Nova Fluor Blue 510 (right). Data were acquired in the B1 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.