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NovaFluor Blue 610-30S Dye
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| 2 | 488 | 610/20 | 508 | 615 | (in buffer) 5 | flow cytometry |
NovaFluor Blue 610 dyes are available with two different levels of brightness: NovaFluor Blue 610-30S and NovaFluor Blue 610-70S, allowing for differential matching of the dye brightness to the antigen density or abundance, without panel redesign. NovaFluor Blue 610-30S and NovaFluor Blue 610-70S dyes have separate spectral signatures but are difficult to use in the same panel. NovaFluor Blue 610-30S is dimmer than NovaFluor 610-70S.
NovaFluor Blue 610-30S dye can be used to replace PE/Dazzle 594, BD Horizon PE-CF594, or PE-Texas Red dye using the 488 nm blue laser line for excitation. As compared with these PE tandem dyes, NovaFluor Blue 610-30S dye shows much less cross-excitation and lower spectral spillover with the 561 nm yellow-green laser line, allowing detection of an additional marker labeled with, for example, NovaFluor Yellow 610 dye. The macromolecule-based NovaFluor Blue 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Lower intensity allows it to be paired with highly expressed antigens
- Can serve as a replacement for PE-tandem dyes using the blue laser, with reduced cross-laser excitation and fluorescent spillover
- Single PE-tandems can be replaced by NovaFluor Blue 610-30S and NovaFluor Yellow 610 dyes to allow detection of an additional marker in a flow cytometry panel
- NovaFluor Blue 610-30S and NovaFluor Blue 610-70S can be used together and unmixed using a spectral flow cytometer
- Produces highly stable fluorescence and is compatible with all buffers tested
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 610-30S dye.
Figure 2. Spectral signature of NovaFluor Blue 610-30S dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 610-30S dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 610-30S (right). Data were acquired in the B6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor dyes
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Cy™ 为 GE Healthcare 的商标或注册商标。
不得转售。Super Bright 聚合物染料依据 Becton, Dickinson and Company 的许可销售。
Brilliant Ultra Violet™ 与 Brilliant Violet™ 为 Becton, Dickinson and Company 或其附属公司的商标或注册商标，经许可使用。
仅供科研使用。不可用于诊断目的。