PE-eFluor 610 染料 | Thermo Fisher Scientific

PE-eFluor 610 spectra excitation shown as dotted line and emission shown as solid yellow histogram


3	488, 532, 561	610/20	565	606	(in buffer) 1	flow cytometry

Invitrogen PE-eFluor 610 dye is a high-performance tandem fluorophore designed for use with flow cytometers equipped with blue (488 nm), green (532 nm), or yellow-green (561 nm) laser. It has a peak emission of 607 nm that can be detected using typical filters for PE-Texas Red dye, such as a 610/20 bandpass filter.

We offer PE-eFluor 610 dye conjugated to a variety of antibodies, and amplification substrates optimized for cellular labeling and detection.

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Benefits of PE-eFluor 610 dye

Brighter than PE-Texas Red dye antibody conjugates
Reduced compensation out of other far-red channels
Stable performance when treated with formaldehyde fixation or exposed to ambient light

Experimental data using PE-eFluor 610 dye

Flow histogram comparing PE-eFluor 610 dye to PE-Texas Red dye

Comparison of staining performance of PE-eFluor 610 to PE-Texas Red dye. Normal human peripheral blood cells (PBMCs) were stained with CD3 Monoclonal Antibody (UCHT1), PE-eFluor 610 (dark orange line) or CD3 Monoclonal Antibody (7D6), PE-Texas Red (light orange line).

Flow histogram showing fluorescence intensity of PE-eFluor 610 dye before and after extended ambient light exposure

Photostability of PE-eFluor 610 dye. Normal human peripheral blood cells (PBMCs) were stained with CD3 Monoclonal Antibody (UCHT1), PE-eFluor 610. Cells were then washed and incubated for six hours at 4°C in the dark (red line), at room temperature in ambient light (blue line), or at room temperature on the windowsill (orange line).