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NovaFluor Blue 745 Dye
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| 2 | 488 | 738/23 | 492 | 750 | (in buffer) 4 | flow cytometry |
Invitrogen NovaFluor Blue 745 dye is a Blue-fluorescent dye with a unique emission spectrum when excited by the 488 nm laser. It fits in space between NovaFluor Blue 725 and NovaFluor Blue 760, a spectral space that is currently not accessible by other Invitrogen spectral dyes potentially allowing the detection of an additional marker in a complex multicolor panel.
NovaFluor Blue 745 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 745 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.
Additional products
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Should be paired with medium to highly expressed antigens to minimize spillover
- Spectrally unique and can be used to fill unused detectors in conventional flow cytometry
- Produces stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Blue 745 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 745 were used for analysis. Data were acquired on a 5-laser Cytek Aurora Flow Cytometer. Signature was normalized to the peak intensity and corrected by removal of the negative-population MFI.
Click image to enlarge
NovaFluor Blue 745 dye is brighter than FITC, dimmer than APC, and compatible with Brilliant dyes. Human PBMC were stained with CD8a Brilliant Violet™ 480 and CD4 (clone SK3) APC (left), CD4 (clone SK3) FITC (middle), or CD4 (clone SK3) NovaFluor Blue 745 dye. Viable cells in the lymphocyte gate were used for analysis, and CellBlox Plus Blocking Buffer was used when staining with NovaFluor Blue 745 dye. Data was collected on a 5-laser Cytek Aurora Flow Cytometer.
NovaFluor dyes
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.