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NovaFluor Yellow 730 Dye
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| 3 | 561 | 695/40 | 551 | 730 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Yellow 730 dye can be used as an alternative to PE-Cyanine 5.5, with far less cross-laser excitation and spectral spillover. NovaFluor Yellow 690, NovaFluor Yellow 700 and NovaFluor Yellow 730 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signature allows them to be successfully unmixed using a spectral flow cytometer.
The macromolecule-based NovaFluor Yellow 730 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Bright fluorescence allows it to be paired with medium-to-low abundance antigens
- Can serve as a replacement for PE-Cy5.5, with far less cross-laser excitation and spectral spillover
- Can be used together with NovaFluor Yellow 690 and NovaFluor Yellow 700 dyes using a spectral flow cytometer
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Yellow 730 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 730 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Yellow 730 dye conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 730 (right). Data were acquired in the YG7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Cy™ 为 GE Healthcare 的商标或注册商标。
不得转售。Super Bright 聚合物染料依据 Becton, Dickinson and Company 的许可销售。
Brilliant Ultra Violet™ 与 Brilliant Violet™ 为 Becton, Dickinson and Company 或其附属公司的商标或注册商标，经许可使用。
仅供科研使用。不可用于诊断目的。
Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标，经许可使用。
仅供科研使用，不可用于诊断目的。