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| 3 | 488/532 | 593/40 | 554 | 566 | (in buffer) 4 (in antifade) 4 | microscopy; flow cytometry |
Invitrogen Cyanine3 (Cy3) dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. We offer a range of Cy3 dye and DiI products including secondary antibodies, streptavidin conjugates, kits, and more.
Invitrogen Alexa Fluor 555 dye is a Cy3 dye alternative that is generally more fluorescent and more photostable than Cy3 dye, making it well suited for applications such as imaging and flow cytometry.
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Figure 1. Double labeling of tubulin and HA-tagged tubulin in T. thermophila using Cy3-goat anti-rabbit and FITC-goat anti-mouse.
Cy™ 为 GE Healthcare 的商标或注册商标。
不得转售。Super Bright 聚合物染料依据 Becton, Dickinson and Company 的许可销售。
Brilliant Ultra Violet™ 与 Brilliant Violet™ 为 Becton, Dickinson and Company 或其附属公司的商标或注册商标,经许可使用。
仅供科研使用。不可用于诊断目的。

