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Qdot 525 Probe
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| 5 | 350 or 633 | 695/40 | 300 | 702 | (in buffer) 5 | microscopy, flow cytometry |
Qdot 525 probe is an excellent green-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~525 nm, Qdot 525 probe can be excited using a 488 nm laser line or standard FITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 525 filter or light cube.
Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also ideal for multiplexed western blots.
Thermo Fisher Scientific offers Qdot 705 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.
Qdot Label Conjugates for Cell and Tissue Staining Qdot 525 secondary antibodies
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Three-color staining of HeLa cells using fluorescent Qdot nanocrystal conjugates. The intracellular structures in fixed HeLa cells were visualized using a red-fluorescent F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot 655 (nuclei), a yellow-fluorescent F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 585 (Golgi), and a green-fluorescent Qdot 525 Streptavidin Conjugate (microtubules).
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
