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Raw Materials and OEM Support for Molecular Assay Development
Move your molecular assay from concept to commercial supply
Developing a molecular assay takes more than selecting a reagent. Thermo Fisher Scientific combines a broad portfolio of molecular biology raw materials with OEM expertise to help support reagent selection, assay optimization, customization, manufacturing, and commercial supply. Our offerings include:
- PCR and qPCR enzymes, reverse transcriptases, DNA polymerases, dNTPs, modifying enzymes, isothermal amplification reagents, buffers, and supporting materials.
- Access to off-the-shelf bulk reagents or OEM specialists to tailor products for sample type, concentration, composition, stability, and workflow requirements.
- Technical and manufacturing support to help your program progress from assay development toward scale-up and commercialization.
Support across your OEM assay development journey
 1. Select |  2. Customize |  3. Scale |
|---|---|---|
| Identify raw materials and reagents that fit your PCR, qPCR, RT-qPCR, isothermal amplification, or NGS workflow. | Adapt reagent concentration, composition, format, and stability requirements to your assay and workflow needs. | Access large-scale manufacturing, quality control, kit assembly, packaging, labeling, and commercial supply support. |
Find raw materials for your molecular assay workflow
Start with the reagent category that matches your application, then use the detailed selection tables to compare products and application compatibility.
Reverse transcriptases
One-step and two-step RT-qPCR, RT-PCR, LAMP, and cDNA synthesis.
DNA polymerases
PCR, qPCR, and one-step RT-qPCR, including hot-start and high-fidelity options.
dNTPs
Individual nucleotides and mixes for amplification and cDNA workflows.
Isothermal amplification
Reagents for LAMP, RPA, RCA, NASBA, TMA, and related workflows.
Modifying enzymes
Enzymes supporting cloning, NGS library preparation, labeling, nucleic acid preparation, IVT, PCR, and more.
Buffers & supporting reagents
Reaction buffers, water, BSA, DTT, ladders, and other molecular assay components.
Featured for fast qPCR and one-step RT-qPCR assay development
Accelerate qPCR assay development with AmpliTaq Ultra DNA Polymerase
Applied Biosystems AmpliTaq Ultra DNA Polymerase is an antibody-based hot-start DNA polymerase designed to help assay developers reduce time to results, build robust qPCR workflows, and maintain flexibility as assay requirements evolve.
30 sec
Polymerase activation
19 min
Demonstrated fast qPCR protocol
Multiplex
Demonstrated DNA and RNA target detection
Why choose AmpliTaq Ultra DNA Polymerase?
- Get results faster with 30-second activation and a fast-cycling protocol demonstrated at 19 minutes on standard qPCR instruments.
- Build robust assays with increased tolerance to common qPCR inhibitors, supporting challenging samples and streamlined workflows.
- Transition existing assays more easily with compatibility across standard Taq buffer systems and demonstrated performance across 10–100 mM KCl.
- Detect more targets per reaction with demonstrated 4-plex one-step RT-qPCR and 5-plex DNA/RNA target detection.
- Gain format flexibility with Lyo-ready and Dry-Ready options for lyophilization, drying, microfluidic, and evolving assay-format needs.
- Simplify logistics with room-temperature storage capability and ambient-temperature shipment.
Extend your OEM journey into NGS library preparation
For customers developing next-generation sequencing workflows, explore raw materials and reagents for NGS library preparation alongside OEM customization and commercial supply support. Thermo Fisher offers products and expertise across key library preparation steps, helping teams build workflows for life science research and molecular diagnostics development.
 Workflow coverage |  Flexible solution |  OEM support |
|---|---|---|
| Products support cDNA synthesis, fragmentation/end repair, adapter ligation, and library amplification. | Options include tailored solutions and lyo-ready enzymes for evolving workflow and stability needs. | Support can span concept development, customization, manufacturing, kit assembly, packaging, and commercial supply. |
Select reagents by application
Table 1. Reverse transcriptases available for assay development.
| Enzymes | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR
| One-step RT-PCR | LAMP | cDNA synthesis | |
| Lyo-ready SuperScript III Flash Reverse Transcriptase | ✓ | – | – | ✓ | – |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, One-step RT-qPCR | ✓ | – | – | – | – |
| Invitrogen SuperScript IV Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Invitrogen SuperScript III Reverse Transcriptase | ✓ | – | ✓ | – | – |
| Thermo Scientific Maxima Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific Maxima H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 2. DNA polymerases available for assay development.
| Enzymes | Applications | ||
|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | PCR | |
| Invitrogen Platinum II Taq Hot-Start DNA polymerase | ✓ | ✓ | ✓ |
| Applied Biosystems AmpliTaq Ultra DNA Polymerase | ✓ | ✓ | ✓ |
| Invitrogen Platinum Taq DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold 360 DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific LibertyTaq DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific Taq DNA Polymerase, recombinant | ✓ | ✓ | ✓ |
| Invitrogen Platinum SuperFi II DNA Polymerase | – | – | ✓ |
| Invitrogen Platinum SuperFi DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Plus DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Hot Start II DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion U Hot Start DNA Polymerase | – | – | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction | |||
View the enzyme selection guide to select products suitable for your application
Table 3. Modifying enzymes available for assay development.
View the enzyme selection guide to select products suitable for your application
Table 4. dNTPs available for assay development.
| Products | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | LAMP | cDNA synthesis | ||
| Thermo Scientific dNTP Mix |
| ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific dATP Solution (100mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dCTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dGTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dTTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dUTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 5. Enzymes for isothermal nucleic acid amplification (INAAT).
WGA: RPA: Recombinase polymerase amplification
| Product | Application |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Lyo-ready Bst DNA Polymerase | LAMP, HDA, EXPAR, RCA, RPA |
| Invitrogen SuperScript IV RT-LAMP Master Mix | LAMP |
| Invitrogen SuperScript IV Reverse Transcriptase and other RTs | LAMP, NASBA, TMA |
| Thermo Scientific T7 RNA Polymerase | NASBA, TMA |
| RNase inhibitors (Ribolock, RNaseOUt, and others) | LAMP, NASBA, TMA |
| RNase H | NASBA |
| Thermo Scientific Phi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific Klenow Fragment, exo– | SDA |
| EXPAR: Exponential amplification reaction HDA: Helicase-dependent amplification LAMP: Loop-mediated isothermal amplification; MDA: Multiple displacement amplification NASBA: Nucleic acid sequence-based amplification RCA: Rolling circle amplification SDA: Strand displacement amplification TMA: Transcription-mediated amplification WGA: Whole-genome amplification RPA: Recombinase polymerase amplification | |
Table 1. Reverse transcriptases available for assay development.
| Enzymes | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR
| One-step RT-PCR | LAMP | cDNA synthesis | |
| Lyo-ready SuperScript III Flash Reverse Transcriptase | ✓ | – | – | ✓ | – |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, One-step RT-qPCR | ✓ | – | – | – | – |
| Invitrogen SuperScript IV Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Invitrogen SuperScript III Reverse Transcriptase | ✓ | – | ✓ | – | – |
| Thermo Scientific Maxima Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific Maxima H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 2. DNA polymerases available for assay development.
| Enzymes | Applications | ||
|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | PCR | |
| Invitrogen Platinum II Taq Hot-Start DNA polymerase | ✓ | ✓ | ✓ |
| Applied Biosystems AmpliTaq Ultra DNA Polymerase | ✓ | ✓ | ✓ |
| Invitrogen Platinum Taq DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold 360 DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific LibertyTaq DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific Taq DNA Polymerase, recombinant | ✓ | ✓ | ✓ |
| Invitrogen Platinum SuperFi II DNA Polymerase | – | – | ✓ |
| Invitrogen Platinum SuperFi DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Plus DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Hot Start II DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion U Hot Start DNA Polymerase | – | – | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction | |||
View the enzyme selection guide to select products suitable for your application
Table 3. Modifying enzymes available for assay development.
View the enzyme selection guide to select products suitable for your application
Table 4. dNTPs available for assay development.
| Products | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | LAMP | cDNA synthesis | ||
| Thermo Scientific dNTP Mix |
| ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific dATP Solution (100mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dCTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dGTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dTTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dUTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 5. Enzymes for isothermal nucleic acid amplification (INAAT).
WGA: RPA: Recombinase polymerase amplification
| Product | Application |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Lyo-ready Bst DNA Polymerase | LAMP, HDA, EXPAR, RCA, RPA |
| Invitrogen SuperScript IV RT-LAMP Master Mix | LAMP |
| Invitrogen SuperScript IV Reverse Transcriptase and other RTs | LAMP, NASBA, TMA |
| Thermo Scientific T7 RNA Polymerase | NASBA, TMA |
| RNase inhibitors (Ribolock, RNaseOUt, and others) | LAMP, NASBA, TMA |
| RNase H | NASBA |
| Thermo Scientific Phi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific Klenow Fragment, exo– | SDA |
| EXPAR: Exponential amplification reaction HDA: Helicase-dependent amplification LAMP: Loop-mediated isothermal amplification; MDA: Multiple displacement amplification NASBA: Nucleic acid sequence-based amplification RCA: Rolling circle amplification SDA: Strand displacement amplification TMA: Transcription-mediated amplification WGA: Whole-genome amplification RPA: Recombinase polymerase amplification | |
Need help selecting or customizing reagents for your molecular assay?
Connect with our OEM team to discuss reagent selection, customization, manufacturing, and commercial supply.
FAQs: Raw materials for custom assay development
Q: Can High Fidelity enzymes be used with TaqMan probes (qPCR application)?
No, High Fidelity enzymes are not compatible with TaqMan probes qPCR. Proofreading DNA polymerases lack 5’→ 3’-exonuclease activity which is essential for TaqMan assay.
Q: What is the best Taq polymerase to use in inhibitor-containing samples in qPCR applications?
Platinum II Taq Hot-Start DNA Polymerase works best with inhibitor-containing samples.
Q: Which reverse transcriptase is the most suitable for developing a one-step RT-qPCR assay?
We recommend using Lyo-ready SuperScript Reverse Transcriptase 1-step RT-qPCR, which was specially created for one-step RT-qPCR application. We have prepared an optimized protocol for a one-step RT-qPCR application that contains Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR, and Lyo-ready Platinum II Taq Hot-Start DNA Polymerase.
Read application note: SARS-CoV-2 RNA detection with the lyo-ready one-step RT-qPCR system
View the user guide: Lyo-ready one-step RT-qPCR system
Q: Is SuperScript IV Reverse Transcriptase suitable for one-step RT-qPCR?
We do not recommend using SuperScript IV reverse transcriptase for one-step RT-qPCR assay due to the high affinity to the template. This feature for one-step RT-qPCR application is excessive because it might prevent polymerase to act efficiently. SuperScript IV Reverse Transcriptase is the number one enzyme of choice for the NGS library preparation step as well as the two-step RT-qPCR assay.
Q: Can Collibri Library Cleanup beads be used instead of AMPure XP and similar products?
Yes, Collibri Cleanup Beads and AMPure XP are interchangeable but note the differences in the cleanup/size selection protocols.
Q: Are Thermo Fisher DNA/RNA modifying enzymes tested in NGS applications?
Yes, all Thermo Fisher DNA/RNA modifying enzymes are rigorously tested and suitable for NGS library prep workflows.
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Stylesheet for Classic Wide Template adjustments